Recombinant Murine IFN-γ 4 ReviewsSubmit a Review Product Details Catalogue Number: 315-05 Description: IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant Murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues. Source: E.coli Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF AA Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg. Calculated Molecular Weight: 15.6 kDa Accession Number: P01580 Gene ID: 15978 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Cow, Human, Human + Mouse, Mouse, Mouse + Chicken, Mouse + Parasite, Rabbit, Rat, Vole References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Cancer Diabetes/Weight Regulation Immune System Neurobiology Stem Cells & Differentiation Allergy Transplantation product.subtitle.recentcitations First Author Kieckhöfer, E Title Primary cilia suppress Ripk3-mediated necroptosis. Citation Cell Death Discovery; 8(1) pg477 PubMed Id 36460631 First Author Kang, T G Title Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx. Citation Nature Communications; 13(1) pg3155 PubMed Id 35672321 First Author Greene, C J Title Macrophages disseminate pathogen associated molecular patterns through the direct extracellular release of the soluble content of their phagolysosomes. Citation Nature Communications; 13(1) pg3072 PubMed Id 35654768 product.subtitle.productreviews Existing reviews Recombinant Murine IFN-γ This product Recombinant Murine IFN-γ was used successfully for our signaling in vitro experiments for the use of CD4, CD8, macrophages as well as the expression of INF-γ. Cells used were mouse lymphocytes isolated from the mouse spleen. From: Linda | Date: 08/07/2020 × Recombinant Murine IFN-γ This product Recombinant Murine IFN-γ was used successfully for our signaling in vitro experiments for the use of CD4, CD8, macrophages as well as the expression of INF-γ. Cells used were mouse lymphocytes isolated from the mouse spleen. From: Linda | Date: 08/07/2020 Close Recombinant Murine IFN-γ Works well for priming our bone-marrow derived macrophages From: MCBL | Date: 07/08/2020 × Recombinant Murine IFN-γ Works well for priming our bone-marrow derived macrophages From: MCBL | Date: 07/08/2020 Close Best cytokine of its kind Have been using it for a long time for our in-vivo pump experiments and it has been a breeze working with this product. It never fails to perform. From: Vineet | Date: 10/08/2020 × Best cytokine of its kind Have been using it for a long time for our in-vivo pump experiments and it has been a breeze working with this product. It never fails to perform. From: Vineet | Date: 10/08/2020 Close Great consistent cytokine for differentiation of Th1 cells and M1 macrophages We routinely use this cytokine to generate mouse CD4 Th1 cells and M1 polarized macrophages. The pricing and consistency of this cytokine can't be beat, and we have been using it for almost 20 years. All of our cytokines used in our lab are from peprotech. From: Chirag | Date: 31/08/2020 × Great consistent cytokine for differentiation of Th1 cells and M1 macrophages We routinely use this cytokine to generate mouse CD4 Th1 cells and M1 polarized macrophages. The pricing and consistency of this cytokine can't be beat, and we have been using it for almost 20 years. All of our cytokines used in our lab are from peprotech. From: Chirag | Date: 31/08/2020 Close
