Growth Factors & Cytokines

Recombinant Murine IFN-γ

Product Details

Catalogue Number: 315-05
Description:

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.  Recombinant Murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues.

Source: E.coli

Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF

AA Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

Calculated Molecular Weight: 15.6 kDa

Accession Number: P01580

Gene ID: 15978

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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Existing reviews
Recombinant Murine IFN-γ
This product  Recombinant Murine IFN-γ was used successfully for our signaling in vitro experiments for the use of CD4, CD8, macrophages as well as the expression of INF-γ.  Cells used were mouse lymphocytes isolated from the mouse spleen.
From:  Linda | Date:  08/07/2020
Recombinant Murine IFN-γ
Works well for priming our bone-marrow derived macrophages
From:  MCBL | Date:  07/08/2020
Best cytokine of its kind
Have been using it for a long time for our in-vivo pump experiments and it has been a breeze working with this product. It never fails to perform.
From:  Vineet | Date:  10/08/2020
Great consistent cytokine for differentiation of Th1 cells and M1 macrophages
We routinely use this cytokine to generate mouse CD4 Th1 cells and M1 polarized macrophages. The pricing and consistency of this cytokine can't be beat, and we have been using it for almost 20 years. All of our cytokines used in our lab are from peprotech.
From:  Chirag | Date:  31/08/2020