Animal-Free Recombinant Murine M-CSF 0 ReviewsSubmit a Review Product Details Catalog Number: AF-315-02 Description: M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of murine M-NFS-60 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 36.4 kDa Accession Number: P07141 Gene ID: 12977 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Immune System Stem Cells & Differentiation Recent Citations First Author Li, X Title Caspase-8 auto-cleavage regulates programmed cell death and collaborates with RIPK3/MLKL to prevent lymphopenia. Citation Cell Death and Differentiation; 29(8) pg1500-1512 PubMed ID 35064213 First Author Hooper, K M Title V-ATPase is a universal regulator of LC3-associated phagocytosis and non-canonical autophagy. Citation The Journal of Cell Biology; 221(6) PubMed ID 35511089 First Author Chakrabarti, J Title Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium. Citation NPJ Regenerative Medicine; 7(1) pg3 PubMed ID 35022438