Recombinant Human GDNF 3 ReviewsSubmit a Review Product Details Catalogue Number: 450-10 Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 crossreactivity: Bird, Frog, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Truvé, K Title Identification of candidate genetic variants and altered protein expression in neural stem and mature neural cells support altered microtubule function to be an essential component in bipolar disorder Citation Translational Psychiatry; 10(1) pg390 PubMed Id 33168801 First Author Bayó-Puxan, N Title Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons. Citation Scientific Reports; 8(1) pg16644 PubMed Id 30413728 First Author Tan, Z Title Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis. Citation Scientific Reports; 8(1) pg16618 PubMed Id 30413738 product.subtitle.productreviews Existing reviews High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 26/08/2020 × High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 26/08/2020 Close Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 24/08/2021 × Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 24/08/2021 Close iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 25/08/2021 × iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 25/08/2021 Close
