Neurotrophins

Recombinant Human GDNF

Product Details

Catalogue Number: 450-10
Description:


Recombinant Human GDNF

GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.

Source: E.coli

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 30.4 kDa

Accession Number: P39905

Gene ID: 2668

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Truvé, K
Title
Identification of candidate genetic variants and altered protein expression in neural stem and mature neural cells support altered microtubule function to be an essential component in bipolar disorder
Citation
Translational Psychiatry; 10(1) pg390
PubMed Id
First Author
Bayó-Puxan, N
Title
Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons.
Citation
Scientific Reports; 8(1) pg16644
PubMed Id
First Author
Tan, Z
Title
Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.
Citation
Scientific Reports; 8(1) pg16618
PubMed Id

product.subtitle.productreviews

Existing reviews
High quality product
Used for human stem cells. Awesome product!
From:  Danielle | Date:  26/08/2020
Cortical Neuron Differentiation
Very consistent for culturing human stem cells
From:  Danielle | Date:  24/08/2021
iPSC-derived neurons
Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons.
From:  Mandi | Date:  25/08/2021