Recombinant Human GDNF 3 ReviewsSubmit a Review Product Details Catalogue Number: 450-10 Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 crossreactivity: Bird, Frog, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Cell Culture Immune System Neurobiology Stem Cells & Differentiation TGF-β Superfamily product.subtitle.recentcitations First Author Sabate-Soler, S Title Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality. Citation Glia; 70(7) pg1267-1288 PubMed Id 35262217 First Author Fanning, S Title Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy. Citation NPJ Parkinson's disease; 8(1) pg74 PubMed Id 35680956 First Author Hanuscheck, N Title Interleukin-4 receptor signaling modulates neuronal network activity. Citation The Journal of Experimental Medicine; 219(6) PubMed Id 35587822 product.subtitle.productreviews Existing reviews High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 26/08/2020 × High quality product Used for human stem cells. Awesome product! From: Danielle | Date: 26/08/2020 Close Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 24/08/2021 × Cortical Neuron Differentiation Very consistent for culturing human stem cells From: Danielle | Date: 24/08/2021 Close iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 25/08/2021 × iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. From: Mandi | Date: 25/08/2021 Close
